Protest all over Italy started after the implementation of restrictions to control the second wave of coronavirus on Monday.
In the northern states of Milan and Turin, where petrol bombs were hurled at officers, clashes were reported.
Tear gas was used in Milan to disperse protestors, and dozens of people gathered in the middle of Naples.
The protests began shortly after the decision to close eateries, pubs, gyms and cinemas by the national government came into force at 18:00 local time.
Night-time curfews have also been enforced in several regions-including Lombardy, where Milan is, and Piedmont, where Turin is. Extremist agitators were blamed for the unrest and cops said 28 people had been detained in Milan alone.
In around a few other city areas, including Rome, Genoa, Palermo and Trieste, demonstrations have taken place. A central square in Turin was held by taxi drivers when restaurant owners smash pots and pans in the middle of Cremona.
In Naples, in central Piazza del Plebiscito, large number of people gathered and demanded the resign from regional governor.
During the beginning of this year there was little opposition to the initial national lockdown. However, the notification of the revived policies has faced immediate public backlash.
Smaller firms say that they are still struggling from the very first lockdown, and that they may be bankrupted by further restrictions.
After the laws came into effect, a variety of luxury shops in central Turin, such as a Gucci boutique, were raided by protesters that spilled into the streets.
Dozens of protesters let firecrackers go off and ignited flares, whilst police counter with tear gas in riot gear.
In Milan, the protesters were shouting “Freedom , freedom, freedom!” as they fought in the city centre with the security forces.